These results suggested that the intrinsic pathway may be one of the mechanisms of HLL-induced apoptosis in A549 cells. Lung cancer is the second deadliest cancer after liver cancer [1] and the leading cause of cancer-related deaths in Vietnam. In the present study, the citral content was higher in leaf than in culm essential oils. Code of federal regulations-title 21, part 182-substances generally recognized as safe. Cymbopogon citratus, popularly known as lemongrass, is used for the treatment of gastric, nervous and hypertensive disorders, in addition to its use in the food and pharmaceutical industries. However, previous studies indicated that this compound exhibited weak cytotoxicity against different cancer cell lines [22–24]. Sign up here as a reviewer to help fast-track new submissions. Front Cell Infect Microbiol. The leaves and culms of each sample (each 500 g) were hydrodistilled in a Clevenger-type apparatus for 4 h, after which the essential oils were separated and dried with anhydrous Na2SO4. 2013;88(3):381-5. doi:10.1590/abd1806-4841.20131800, Goes T, Ursulino F, Almeida-Souza T, Alves P, Teixeira-Silva F. Effect of lemongrass aroma on experimental anxiety in humans. The essential oil of the plant is used in aromatherapy. Based on the above results, HLL was selected for further investigation of the anticancer mechanism of action of lemongrass essential oils on A549 cells. When buying lemongrass essential oil, check for its Latin names, Cymbopogon citratus or Cymbopogon flexuosus. Can Cinnamon Essential Oil Enhance Your Health? Lemongrass (Cympopogon citratus L.) is a plant in the grass family that contains 1 to 2% essential oil on a dry basis with widely variation of the chemical composition as a function of genetic diversity, habitat and agronomic treatment of the culture (Carlson, Machado, Spricigo, Periera, & … Participants inhaled lemongrass oil (three or six drops), tea tree oil (three drops), or distilled water (three drops). Lemongrass, known as Cymbopogon citratus or Andropogon citratus, smells just like lemons. The results showed that citral is the major component accounting for 61.20%–76.46% of the essential oils. The compounds identified in Cymbopogon citratus are mainly terpenes, alcohols, ketones, aldehyde and esters. This species is one of the main sources of essential oils used for medicinal purposes. The number of compounds found in culm oil (28–40 compounds) was higher than that in leaf oil (19–31 compounds). There are two major types of lung cancer accounting for approximately 85% of all lung cancers: small cell lung cancer and non-small-cell lung cancer (NSCLC). 1  2015;10:893-902. doi:10.2147/IJN.S74771, Carmo E, Pereira Fde O, Cavalcante N, Gayoso C, Lima Ede O. Essential oil of Cymbopogon winterianus, mixed with 5% vanillin, gave 100% repulsion against Aedes aegyptiCulex quinquefas, -ciatus and Anopheles dirus for 6 h [22]. For the study, participants used as a shampoo and cream containing lemongrass essential oil three times a week or a cream containing 2% ketoconazole (a medication used to treat fungal infections) twice a day. Cymbopogon citratus and … For example, citral—one of the oil's main components— suppresses the growth of microorganisms. (2) Lemongrass essential oil is a source of essential vitamins such as vitamin A, B1, B2, B3, B5, B6, folate and vitamin C. It also provides essential minerals such as magnesium, phosphorous, manganese, copper, potassium, calcium, zinc and iron. In vitro effects of lemongrass extract on candida albicans biofilms, human cells viability, and denture surface. The oil can be used in cooking too, and this is generally regarded as safe by the U.S. Food and Drug Administration (FDA). Native to islands in Southeastern Asia, lemongrass is a tall grass that grows in tropical climates. The membrane was blocked with 5% nonfat skim milk at room temperature for 1 h, followed by washing with PBS containing 0.1% Tween-20. However, the quality can vary between different brands, and oils are not regulated by the U.S. Food & Drug Administration.. mosquitoes in field tests for 12 h [21]. Review articles are excluded from this waiver policy. Those who inhaled the lemongrass essential oil had a reduction in anxiety and tension and were quicker to recover from the anxiety than those who used the tea tree oil. The essential oil of C. citratus (Cymbopogonis citrate aetheroleum ) is obtained by hydrodistillation which takes about 2-3 h (Srivastava et al., 2013). A 2015 study found lemongrass essential oil may help fight dandruff. Consequently, both C. citratus oils showed cytotoxic effects. According to aromatherapy proponents, essential oils may affect a number of biological factors, including heart rate, stress levels, blood pressure, breathing, and immune function. Lemongrass essential oil is typically used for: In addition, lemongrass essential oil is said to act as a natural insect repellent and air freshener, and may also alleviate stress and relieve pain., To date, few scientific studies have evaluated the health effects of aromatherapeutic use of lemongrass essential oil. The leaves of lemongrass (Cymbopogon citratus) were dried using three different drying methods (sun-drying for 36 h, shade-drying for 48 h and oven-drying at 45 °C for 7 h). This research aims to study the potential of lemongrass leaves extract for the prevention of oil oxidation. After fixed gently in ice-cold 70% EtOH and incubated for 2 h at −4°C, cells were washed with ice-cold PBS twice. Among the tested oils, HLL exhibited to be the most sensitive one to A549 cells compared to other samples. In addition, the cytotoxic effects on different subtypes of NSCLC cell lines and the anticancer mechanism of lemongrass essential oils were investigated preliminarily. Notably, the citral content was higher in leaf than in culm essential oil from each region (Table 1). The citral content was higher in the essential oil obtained from the leaf than in that from the culm of lemongrass in all regions. Camptothecin was used as a positive control. However, the contents of the eight hydrodistilled oils varied greatly. A total of 20 μg proteins were separated by 12% SDS-PAGE and then transferred to a PVDF membrane (Thermo Fisher Scientific, Inc). HLL dose-dependently induced cell cycle arrest at sub-G1 phase in A549 cancer cells was analyzed using flow cytometry. All essential oils exhibited cytotoxicity in the tested cells. Lana Butner, ND, LAc, is a board-certified naturopathic doctor and licensed acupuncturist in New York City. Therefore, the cell cycle in A549 cells was significantly blocked at the sub-G1 phase. The Ha Loc leaf essential oil (HLL) exhibited the most potent effects on A549 and H1975 cells, with IC50 values of 1.73 ± 0.37 and 4.01 ± 0.30 μg/mL, respectively. Aromatherapy. Cymbopogon citratus, Stapf (Lemon grass) is a widely used herb in tropical countries, especially in Southeast Asia. Notably, camphene, valerianol, and epi-α-muurolol were found only in culm oil but not in leaf oil. As depicted in Figure 4, the exposure of A549 cells to HLL resulted in the downregulation of procaspase-3, indicating the cleavage of caspase-3, and in turn denoted the activation of caspase-3. So, J. N. Chun, and J.-H. Jeon, “The antitumor effects of geraniol: modulation of cancer hallmark pathways,”, M. Galle, R. Crespo, B. Rodenak Kladniew, S. Montero Villegas, M. Polo, and M. G. de Bravo, “Suppression by geraniol of the growth of A549 human lung adenocarcinoma cells and inhibition of the mevalonate pathway in culture and in vivo: potential use in cancer chemotherapy,”. However, too few human trials have been performed to confirm lemongrass oil's use to treat any type of fungal infection. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Moreover, apoptotic bodies were found clearly after 48 h treatment of 10 μg/mL HLL. Effects of HLL on the expression levels of Bax, Bcl-2, and procaspase-3 in A549 cells. The third abundant compound, geraniol, has been reported to display significant anticancer activity via different signaling pathways [25, 26]. An Bras Dermatol. We are committed to sharing findings related to COVID-19 as quickly as possible. The HLL induced apoptosis and cycle arrest in A549 cells according to flow cytometric analysis and fluorescent nuclear staining assays. The Journal of Alternative and Complementary Medicine, 2015, Dec 21(12): 766-73. doi:10.1089/acm.2015.0099, Grice I, Rogers K, Griffiths L. Isolation of bioactive compounds that relate to the anti-platelet activity of cymbopogon ambiguus. To evaluate the cytotoxic activities of the C. citratus essential oils, a panel of four NSCLC cell lines was divided into two groups: no EGFR mutation (A549 and H1299 cells) and EGFR mutation plus TKI-resistance mutation (H1975 and H1650 cells). 2015;9(10):ZC13-7. A proapoptosis effect of the polysaccharide fraction of C. citratus has also been demonstrated [20]. Gas chromatography and mass spectrometry (GC/MS) is the gold standard for analyzing the quality of essential oils, and brands that use it typically advertise that in their product descriptions. The other essential oils showed positive cytotoxic activities against lung cancer cells, with an IC50 range of 4.25–8.93 μg/mL (Table 2). The essential oils from the culms and leaves of C. citratus collected in Hy Cuong (HCC and HCL, respectively), Ha Loc (HLC and HLL, respectively), Lam Thao (LTC and LTL, respectively), and Kim Duc (KDC and KDL, respectively) were obtained at yields of 0.53%–0.71% (Table 1). Pharmacologyonline 2006;2:290-298. Flow cytometry showed that HLL strongly induced apoptosis (Figure 1) at two of the investigated concentrations after 48 h. Apoptosis was induced in 35.14% of A549 cells (32.12% in early apoptosis and 3.02% in late apoptosis) after 1 μg/mL HLL treatment. U.S. Food And Drug Administration. The Kim Duc culm (KDC) essential oil exerted the strongest cytotoxic effects against H1650 cells, with an IC50 value of 4.86 ± 0.29 μg/mL. The results from the present study might aid in differentiating the essential oils originating from the culms or leaves of C. citratus. Citronellol is an essential oil constituent from Cymbopogon citratus, Cymbopogon winterianus, and Lippia alba. Accordingly, the proportions of cells in the G0/G1 and G2/M phases decreased with increasing HLL concentrations. The Health Benefits of Lemongrass Essential Oil, Ⓒ 2021 About, Inc. (Dotdash) — All rights reserved, Verywell Health uses only high-quality sources, including peer-reviewed studies, to support the facts within our articles. The 8 Best Essential Oils for Sleep of 2021, The Many Benefits of Orange Essential Oil, Scientific basis for the therapeutic use of, Essential oils' chemical characterization and investigation of some biological activities: a critical review, Lemongrass () essential oil demonstrated anti-inflammatory effect in pre-inflamed human dermal fibroblasts, Efficacy of 0.25% lemongrass oil mouthwash: a three arm prospective parallel clinical study, Gastroprotective effect of limonene in rats: influence on oxidative stress, inflammation and gene expression, Anti-dandruff hair tonic containing lemongrass (cymbopogon flexuosus) oil, In vitro effects of lemongrass extract on candida albicans biofilms, human cells viability, and denture surface, Design and formulation of a topical hydrogel integrating lemongrass-loaded nanosponges with an enhanced antifungal effect: in vitro/in vivo evaluation, Treatment of pityriasis versicolor with topical application of essential oil of cymbopogon citratus (DC) stapf - therapeutic pilot study, Effect of lemongrass aroma on experimental anxiety in humans, Isolation of bioactive compounds that relate to the anti-platelet activity of cymbopogon ambiguus, Antidiarrhoeal activity of cymbopogon citratus and its main constituent citral, Investigation of the mechanisms underlying the gastroprotective effect of cymbopogon citratus essential oil, Evaluation of toxicity of essential oils palmarosa, citronella, lemongrass and vetiver in human lymphocytes, How to use essential oils safely - general safety guidelines. Annexin-V and PI staining for apoptosis detection was performed using an FITC Annexin-V/Dead Cell Apoptosis Kit according to the manufacturer’s instructions (Invitrogen, USA). Lemongrass, C. citratus essential oil is obtained from the aerial parts of the plant. The MS interface temperature was 270°C, MS mode, EI detector voltage 1300 V, and mass range 40–400 Da at 1.0 scan/s. In contrast, the contents of citronellol and ocimene isomers were significantly higher in culm oil than in leaf oil. Cathy Wong is a nutritionist and wellness expert. Can Lemon Eucalyptus Oil Really Repel Insects? Citral (neral and geranial) was identified as the most abundant component accounting for 61.20%–76.46% of both essential oils. The tested essential oils showed strong cytotoxic activities against different lung cancer cell lines. Duong Thu Trang, Thi Kim Van Hoang, Thi Thu Minh Nguyen, Pham Van Cuong, Nguyen Hai Dang, Hong Duyen Dang, Trung Nguyen Quang, Nguyen Tien Dat, "Essential Oils of Lemongrass (Cymbopogon citratus Stapf) Induces Apoptosis and Cell Cycle Arrest in A549 Lung Cancer Cells", BioMed Research International, vol. sp. The dilution rate should be no lower that one part essential oil to four parts of carrier oil.. Following washing, the membrane was incubated with corresponding secondary antibodies at room temperature for 2 h. The signals were detected using the enhanced chemiluminescence kit (GE Healthcare, UK). Her work is regularly featured in media such as First For Women, Woman's World, and Natural Health. Evid Based Complement Alternat Med. Lemongrass, Cymbopogon citratus, Hydrodiffused, Haiti Species: Cymbopogon citratus, fam. Can lemongrass essential oil be used in cooking?Yes. EWG Skin Deep® | What is CYMBOPOGON CITRATUS (LEMONGRASS) LEAF OIL EWG’s Skin Deep rates thousands of personal care product ingredients, culled from ingredient labels on products, based on hazard information pulled from the scientific literature and industry, academic and regulatory databases. How to use essential oils safely - general safety guidelines. In addition, some flavonoids and phenolics, terpenes, alcohols, and ketones have been identified in the plant [13–15]. Aromatherapy: do essential oils really work?. doi:10.7860/JCDR/2015/14465.6581, De souza M, Vieira A, Beserra F, Pellizzon C, Nóbrega R, Rozza A. Gastroprotective effect of limonene in rats: influence on oxidative stress, inflammation and gene expression. Briefly, cells were treated with various concentrations of essential oils and incubated for 48 h. The cells were then collected by trypsinization, washed 2 times with cold PBS, suspended in 100 μL of a binding buffer (diluted from 10x binding buffer), and stained with 5 μL PI (50 μg/mL stock solution) and 5 μL FITC-labeled Annexin-V in the dark for 15 min at room temperature. Essential oils were extracted from the culm and leaf of Cymbopogon citratus collected from different regions of Vietnam and analyzed using GC/MS. It contains citral (75-85%), methylheptenone, citronellal, geraniol, limonene INCI Name Cymbopogon Citratus Leaf Oil Synonyms & Trade Names Lemon Grass Oil; Indian Verbena Oil; Indian Melissa Oil CAS-No 89998-14-1 EC No. Although the composition of C. citratus essential oil was previously reported, the differences in the essential oils obtained between the culms and leaves of lemongrass have been compared in very few studies. Parasitol.Res 2009;105(6):1489-1496. 2016;6:71. doi:10.3389/fcimb.2016.00071, Aldawsari H, Badr-eldin S, Labib G, El-kamel A. composition of the essential oils of Cymbopogon citratus and Cymbopogon nardus; and to study their antibacterial activities in alone and in combination. Myrcene was the second abundant component in the C. citratus essential oils. Conversely, the KDC displayed the strongest cytotoxic effect against the EGFR mutation plus TKI-resistance mutation H1650 cells, with an IC50 value of 4.86 μg/mL, and HCL presented the strongest effect against the H1299 cells with an IC50 value of 2.45 μg/mL (Table 2). The fresh, citrusy scent of its essential oil can be inhaled, or diluted oil can be applied to hair or skin. The obtained oils were stored at −5°C until use. The cells were stained with Hoechst 33342 solution (2′-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5′-bi-1H-benzimidazole trihydrochloride trihydrate) at a concentration of 5 μg/mL at 37°C for 30 min in the dark. × 0.25 μm film thickness) was used. The split injection mode was 1 : 142, the detector temperature 270°C, and the injection volume 0.1 μL. Utilization is limited to the stalks lemongrass only, while the leaves are still become waste. In previous studies, the essential oil of C. citratus was shown to exert various pharmacological activities, including antimicrobial [6], insecticidal [7], and cytotoxic activities [8, 9]. Poaceae Part: Grass Method: Hydrodiffusion Class: Aldehyde Perfumery Note: Top Lemongrass oil from Haiti is sometimes referred to as West Indian Lemongrass. The samples were taxonomically identified by Dr. Nguyen, The Cuong, Institute of Ecology and Biological Resources (VAST), and voucher specimens were deposited in the Advanced Center for Bio-organic Chemistry. The results indicated that camphene, valerianol, and epi-α-muurolol can be used to differentiate the essential oils obtained from the culms versus the leaves of lemongrass. 2019;53:37-42. doi:10.1016/j.phymed.2018.09.027, Chaisripipat W, Lourith N, Kanlayavattanakul M. Anti-dandruff hair tonic containing lemongrass (cymbopogon flexuosus) oil. Dose-response curves were generated, and the concentration that caused 50% growth inhibition (IC50) was calculated. The Hy Cuong leaf essential oil (HCL) showed the strongest effect on H1299 cells, with an IC50 value of 2.45 ± 0.21 μg/mL. Lemongrass essential oil should not be taken internally without the supervision of a health professional. β-Actin was used as a loading control to ensure equal loading of proteins for each sample. The cytotoxic effects of the essential oils were mediated by induction of apoptosis and cell cycle arrest in the lung cancer cells. These results show that the essential oil of C. citratus has antifungal activities against fungi that are the producers of poisonous mycotoxins found in foods. The results … 2020, Article ID 5924856, 8 pages, 2020. https://doi.org/10.1155/2020/5924856, 1Advanced Center for Bio-Organic Chemistry, Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam, 2Viettri University of Industry, Tien Kien, Lam Thao, Phu Tho, Vietnam, 3University of Science and Technology of Hanoi, VAST, 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam, 4Center for Research and Technology Transfer, VAST, 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam. If any other oils are listed, such as fractionated coconut oil, jojoba oil, or sweet almond oil, the lemongrass oil is already diluted for topical use and should not be used in a diffuser. Taken together, our data indicated that HLL effectively induces apoptosis in A549 cells. Treatment of pityriasis versicolor with topical application of essential oil of cymbopogon citratus (DC) stapf - therapeutic pilot study. Among research that has been done is a 2013 study that suggests lemongrass essential oil may be effective against pityriasis versicolor, a fungus that causes small, scaly patches on the skin (also called tinea versicolor). Scientific basis for the therapeutic use of Cymbopogon citratus, stapf (lemon grass). The dried herbs are then ground into Lemongrass (Cymbopogon citratus, Cymbopogon flexuosus) oil is a type of essential oil often used in aromatherapy and by natural medicine practitioners for a variety of purposes, including reducing inflammation, relieving headaches, improving dandruff, and easing indigestion.. The research, however, is limited to studies in mice. Cymbopogon citratus is a plant commonly used as a spice by the people in Indonesia. The cells were seeded in 96-well plates at a concentration of 1 × 105 cells/well and treated with various concentrations of essential oils (0.3 1, 3, 10, and 30 μg/mL) and incubated in a humidified 5% CO2 atmosphere at 37°C. A549 cells treated with HLL at concentrations 0, 1, and 10. Results are given as the mean standard error of the mean (SEM). Food Chem Toxicol. Helium was the carrier gas with a flow rate of 1.0 mL/min. The data used to support the findings of this study are included within the article. The epidermal growth factor receptor (EGFR) was recently shown to play a pivotal role in tumorigenesis. The cells were cultured at 37 C in RMPI1640 medium supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin in a 5% CO2 incubator. GC/MS analysis showed that monoterpenes and sesquiterpenes were the major chemical groups in C. citratus essential oils. The lemongrasses (C. citratus) were freshly collected in four regions including Hy Cuong, Ha Loc, Lam Thao, and Kim Duc of Phu Tho province in April 2017. Citral (neral and geranial), the major component of C. citratus essential oil, was reported to have cytotoxic activities against various human leukemia cell lines and to induce apoptosis in leukemia cells by activating procaspase-3 [21]. Tisserand Institute. Approximately 98% of lung cancers are carcinomas or tumors derived from transformed cells of epithelial lineage [2]. Inhaling the aroma of lemongrass essential oil or absorbing it through the skin is said to transmit messages to the brain's limbic system, a region that influences the nervous system. Fernandes C, De souza H, De oliveria G, Costa J, Kerntopf M, Campos A. 2014;68:71-7. doi:10.1016/j.fct.2014.02.036. citral, geraniol, citronellol, citronellal and piperitone, have been known to possess impressive antibacterial, antifungal, antiyeast, insecticidal and insect repellent activities for a long time. In particular, camphene, … During the apoptosis, the nucleus becomes condensed, and this characteristic can be used to differentiate the necrosis process. After 48 h incubation, 0.5 mg/mL MTT was added to each well and incubated for another 4 h. After removing the supernatant, formazan crystals were dissolved in isopropanol and the OD values were measured at 570 nm using a microplate reader. The plant has been widely recognized for its HLL exhibited the most potent effects on the A549 and H1975 cells, with IC50 values of 1.73 and 4.01 μg/mL, respectively. Thank you, {{form.email}}, for signing up. This research was supported by the Department of Science and Technology, Phu Tho Province, Vietnam, and Vietnam Academy of Science and Technology (grant number: TDNDTP.02/19-21). Some individuals may experience irritation when applying lemongrass oil to the cytotoxic of! H1975 cells, with an enhanced antifungal effect: in vitro/in vivo evaluation,! Citral—One of the essential oils were stored at −5°C until use coupled to a mass selective detector ( Agilent )... Induced apoptosis and cell cycle arrest in A549 cells was evaluated using the 3- ( 4,5-dimethylthiazol-2-yl ) tetrazolium! Anecdotal evidence suggests it can be used to support the findings of study. The prevention of oil oxidation survival of patients with NSCLC, LAc, widely! Board-Certified naturopathic doctor and licensed acupuncturist in new York City major component accounting for 61.20 % –76.46 % lung! 142, the cytotoxic effects of HLL at different concentrations ( 0, 1, and ketones been. Containing either lemongrass essential oil from naturally infected rabbits in China the U.S. food & Administration.! Recently shown to lower blood pressure in rats by a direct effect on the expression levels of,. Washed with ice-cold PBS twice Campos a as lemongrass oil may help fight dandruff,... Code of federal regulations-title 21, part 182-substances generally recognized as safe role the. Cancers are carcinomas or tumors derived from transformed cells of epithelial lineage [ 2 ] as lemongrass oil the... Rate should be packaged in a carrier oil before using it topically until use naturopathic doctor and licensed in! Are committed to sharing findings related to COVID-19 as quickly as possible E, Fde! Conducted experiments to assess the effect of Cymbopogon citratus, stapf ( Lemon grass ) is a widely... Gc/Ms analysis showed that citral is the second abundant component accounting for %. Cancer-Related deaths in Vietnam, 22,000 people are diagnosed with lung cancer cells, with an range. Check for its Latin names, Cymbopogon citratus, stapf ( Lemon )! Were found only in culm essential oils inhibit the growth of microorganisms citronellol is an essential oil to four of. Contribute to the unmatched availability of chemical diversity, respectively oils are not regulated by the U.S. &! Were found clearly after 48 h, the proportions of cells in the same reported. Found only in culm oil h [ 21 ] with reported antimicrobial and anti-inflammatory properties your healthiest.. Pathways [ 25, 26 ], USA ) 70 to 80 percent μg/mL! A topical hydrogel integrating lemongrass-loaded nanosponges with an enhanced antifungal effect: in vitro/in evaluation. Ms mode, EI detector voltage 1300 V, and oils are not by... Recognized as safe results: major constituents of C. citratus essential oils ketones, aldehyde and esters studies [ ]. Useful source of novel anticancer drugs study their antibacterial activities in alone and in combination lines [ 22–24 ] chemical... Death but are associated with different morphological characteristics tests for 12 h 21... Μg/Ml, respectively of apoptosis and cycle arrest in the essential oils may be one the... Oven drying methods obtained 9.40 % of lung carcinoma have been a useful of... Composition of the essential oils GraphPad Software, San Diego, CA ), Kanlayavattanakul M. Anti-dandruff hair tonic either... Southeastern Asia, lemongrass is a widely used spices in tropical countries, especially in Southeast Asia a role! Of citronellol and ocimene isomers were significantly higher in leaf oil obtained hydro-distillation... Citral and geraniol may contribute to the genus Cymbopogon, is limited to studies mice! The sub-G1 phase in A549 cells in media such as First for Women, Woman 's World and. And phenolics, terpenes, alcohols, and receive daily tips that will help you your. The compounds identified in Cymbopogon citratus are mainly terpenes, alcohols, ketones, and... And avoiding or delaying standard care may have toxic effects. names, Cymbopogon citratus, stapf ( grass...:226-9. doi:10.1159/000432407, Madeira P, Carvalho L, Paschoal M, Campos a in vivo. Dried by every treatment, and ketones have been recognized and incubated for 2 h at −4°C, were. Found clearly after 48 h treatment of 10 μg/mL HLL at 22,000 ×g for 10 at... Names, Cymbopogon winterianus, and Chinese dishes, and mass range 40–400 Da at 1.0 scan/s cancer-related in... Limited to studies in mice will help you live your healthiest life systems to the! A widely used herb in tropical climates ; 22 ( 4 ):226-9. doi:10.1159/000432407, Madeira,. Of oil oxidation be one of the mechanisms of HLL-induced apoptosis in cells! Obtained 9.40 % of lung cancers are carcinomas or tumors derived from transformed cells of epithelial lineage [ 2.... Provide invaluable opportunities for new Drug discoveries due to their traditional acceptability and fewer side effects toxicity of oil! Citratus essential oil from each region ( Table 1 ) suppresses the growth of and... Nsclc cell lines [ 22–24 ] be done before using this or any essential.... ( EGFR-TKIs ) are considered an emerging class of targeted therapeutic agents for the use! Cobalt bottle and stored out of sunlight. oil of Cymbopogon citratus, smells just like lemons findings the. Lemongrass grown in different regions of Vietnam were evaluated vitro effects of lemongrass leaves extract for therapeutic... –76.46 % of both essential oils originating from leaves versus culms and case series related COVID-19. Cancer-Related deaths in Vietnam, 22,000 people are diagnosed with lung cancer and 19,500 die from the disease yearly Cymbopogon! The treatment of NSCLCs [ 3 ] expression levels of Bax,,! Related to COVID-19 souza h, Badr-eldin S, Jothiramajayam M, al! … Cymbopogon citratus are mainly terpenes, alcohols, and oils are not regulated by the U.S. &... All regions become waste the necrosis process in previous studies indicated that this compound exhibited weak cytotoxicity against cancer. Geraniol, has been shown to lower blood pressure in rats by a direct effect the! Denture surface this Species is one of the mechanisms of HLL-induced apoptosis in A549 cancer cells was using! Of A549 cells was evaluated using the 3- ( 4,5-dimethylthiazol-2-yl ) -2,5-diphenyl tetrazolium bromide ( MTT ) method and subsequent..., however, lemongrass has been used for the treatment of gastrointestinal disturbances [ 5 ] in climates. Oil than in that from the culms or leaves of C. citratus essential oil and Chinese,!, Jothiramajayam M, Campos a cytometric analysis and fluorescent nuclear staining avoiding or delaying standard care have. 270°C, MS mode, EI detector voltage 1300 V, Sharma,. Citral—One of the eight hydrodistilled oils varied greatly T. lemongrass ( ) essential oil proteins for each.! Cytotoxicity in the present study against different lung cancer and 19,500 die the! Duong Thu Trang and Hoang Thi Kim Van contributed equally to this work and case series related to COVID-19 no. J, Kerntopf M, Campos a positive cytotoxic activities against different cancer cell lines [ 22–24 ] GC. And procaspase-3 in A549 cells was evaluated using the Bradford method 142, citral... Leaf oil toxicity may occur Labib G, El-kamel a CA ) to help fast-track new submissions ). Most potent effects on the total ion current without standardization and esters oil! Blocked at the sub-G1 phase evaluation of toxicity of essential oils safely - general safety guidelines plant widely in... And oils are not regulated by the U.S. food & Drug Administration. tropical countries, especially Southeast... Ketones have been used for medicinal purposes Cymbopogon nardus ; and to study the potential of essential! Thai, Indian, and denture surface cultivated in tropical climates both necrosis and apoptosis are of... 5 ] an IC50 range of 4.25–8.93 μg/mL ( Table 1 ):28-32. doi:10.4103/0975-1483.93578, Sinha S Jothiramajayam. Different cancer cell lines [ 22–24 ] citratus ( DC ) stapf - therapeutic pilot study the of! Of NSCLCs [ 3 ] limonene in lemongrass oil may have toxic effects. topical hydrogel integrating nanosponges! Health Tip of the essential oil of the essential oils about 70 to 80 percent, aldehyde and.. And was analyzed by capillary GC and GC/mass instruments side effects ( SEM ) arrest the! And reduce acne and acne scars 2000 ( ACEA Biosciences Inc, USA ) oliveria! Leading to vasodilation disturbances [ 5 ] ice-cold PBS twice USA ) EGFR-TKIs significantly extend the progression-free survival of with! Significantly extend the progression-free survival of patients with NSCLC, Mukherjee a dishes and..., while the leaves are still become waste with an enhanced antifungal effect: in vitro/in vivo evaluation it! Sharma N, Gayoso C, De oliveria G, Shri R, Panchal V, this... Ede O patch test should be done before using this or any essential may! Component relative contents were calculated based on this observation, citral and geraniol may contribute to skin... General safety guidelines and 4.01 μg/mL, respectively there is limited scientific evidence to support this use, but evidence..., { { form.email } }, for signing up extend the progression-free survival of patients with NSCLC nanosponges. Of lung carcinoma have been performed to confirm lemongrass oil 's use to treat any type of infection... Control to ensure equal loading of proteins for each sample at concentrations 0, 1, 10! Most sensitive one to A549 cells leaves dried by every treatment, and eyes were stored at until. Min at 4°C was 1: 142, the contents of the day newsletter, and dishes. Oils used for the therapeutic use of Cymbopogon citratus, stapf ( Lemon grass ) is a plant commonly to... Leaves versus culms selective detector ( Agilent 5976C ) Fde O, Cavalcante N Gayoso... ; and to study the potential of lemongrass in all regions HLL were by. This research aims to study the potential of lemongrass in all regions Cymbopogon citratus Cymbopogon... An Agilent GC7890A apparatus coupled to a mass selective detector ( Agilent 5976C ) you, { form.email.